Fig. 9.

Ablation of kindlin-2 inhibits Kras^G12D-induced lung tumorigenesis and reduces the mortality rate. a–e The lung of the mice (as indicated in the figure) was administrated with Ad-Cre and analyzed 16 weeks later. The gloss morphology of the lung was observed (a). Scale bar = 1 cm. The lung tissues from the mice were weighed (b, 3 mice for each group). The lung was pressed onto two slides and observed under microscopy for analyses of lung tumors (c). Scale bar = 0.5 cm. Sections of the lung tissues from the mice were analyzed by HE staining (d). Scale bar = 200 μm. Tumor area was quantified as described in the Methods (e, 4 mice for each group). f Kaplan–Meier survival curve of Kras^LSL−G12D/+(Kras ^fl/+) mice (n = 14) vs. Kras^LSL−G12D/+; kindlin-2^fl/fl (Kras ^fl/+; K2^fl/fl) mice (n = 11) for up to 428 days post-administration of Ad-Cre. Data in b and e present mean ± SEM (n = 3) using one-way ANOVA with Tukey–Kramer post-hoc analysis, **P < 0.01; ***p < 0.001. Kaplan–Meir survival analysis in f was determined by log-rank test