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Primers of PCR and sequencing for 16S rRNA in this study.
| Primer | Sequence (5′ to 3′) | Amplicon size (bp) | References |
|---|---|---|---|
| PCR of 16S rRNA | |||
| 8 f | CACGGATCCAGACTTTGAT(C/T)(A/C)TGGCTCAG | 1498 | 14 |
| 1512r | GTGAAGCTTACGG(C/T)TAGCTTGTTACGACTT | ||
| Sequencing of 16S rRNA | |||
| 8 f | CACGGATCCAGACTTTGAT(C/T)(A/C)TGGCTCAG | — | 15 |
| 534r | ATTACCGCGGCTGCTGG | ||
| 534 f | CCAGCAGCCGCGGTAAT | ||
| 968 f | AACGCGAAGAACCTTAC | ||
| 1512r | GTGAAGCTTACGG(C/T)TAGCTTGTTACGACTT | ||
