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. 2019 Feb 19;10:846. doi: 10.1038/s41467-019-08728-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Cryo-electron tomography studies of HRPV-6. a A slice through a 3D tomogram of HRPV‐6 reconstructed from tilt series data collected using a Volta phase plate. The scale bar corresponds to 40 nm. b Reconstruction of an HRPV-6 virion. 41 VP5 spikes were found from a representative HRPV-6 virion by manual picking. The EM density was projected onto the aligned positions and orientations of these spikes. c VP5 crystallographic structure fitted into its density solved by cryo-electron tomography and subtomogram averaging