Fig. 6.

HRPV VP5 extended conformation and proposed model. a HRPV-6 virions incubated at room temperature do not show extended spikes. b HRPV-6 virions incubated at 55 °C, showing VP5 in more extended conformation. c A cryo-EM image showing a particle with extended spikes (low salt). Scale bars correspond to 50 nm. d Proposed model for the rearrangement of HRPV spike from the pre-fusion to the membrane insertion step conformation. The N- and C-terminal domains of VP5 are coloured in blue and red, respectively, whereas the putative host recognition (N2) domain is coloured in green. VP5 would bind to the host via its N2 domain and opens up an elongated structure of N1