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. 2019 Feb 19;9:2254. doi: 10.1038/s41598-019-39218-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SP-htau is secreted to the medium of stably transfected SH-SY5Y cells and is N-glycosylated: (a) Dot blot using 5A6 antibody (against total tau) on: medium of SH-SY5Y cells overexpressing SP-htau (top panel, I) and medium of non-transfected SH-SY5Y cells (bottom panel, II). (b) The same cell lines used for the dot blot (left – SH-SY5Y cells overexpressing SP-htau; right – non-transfected SH-SY5Y cells), were lysed and total protein level was evaluated on Western blot, using ab8228 antibody (against β-actin). (c) Western blot using 5A6 antibody (against total tau) on the medium from SH-SY5Y cells overexpressing SP-htau prior to (right) and following (left) PNGase-F treatment.