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. 2019 Feb 19;9:2254. doi: 10.1038/s41598-019-39218-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Medium containing deglycosylated SP-htau exhibits elevated aggregation: Time dependent Thioflavin-T binding kinetics of culture medium from (a) non-transfected SH-SY5Y cells treated (red) or untreated with PNGase-F (black), and of SH-SY5Y cells expressing SP-htau prior to (blue) and following PNGase-F treatment (cyan). (b) Relative ThT signal at the end point of incubation at 37 °C. Non-transfected untreated cells were set as 100%. P-values: ***p < 0.001 and ^###p < 0.001.