Fig. 3.

MST1 regulates nuclear localization of FOXO1 at tip ECs. a Images of CD31⁺ retinal vessels and distribution of FOXO1 of whole retina in WT mouse at P6. The red dashed lines separate into tip ECs, vascular front and vascular plexus from top to bottom. Scale bars, 200 μm. b Magnified images of CD31⁺ vessels and subcellular localization of FOXO1 at indicated portions. Scale bars, 50 μm. c Magnified images of the nuclear localization of FOXO1 (yellow arrowheads) at tip ECs in WT and Mst1^i∆EC mice. Scale bars, 50 μm. d Images of angiopoietin-2 (Angpt2) expression and CD31⁺ vessels at vascular front in WT and Mst1^i∆EC mice. Scale bars, 100 μm. e Comparisons of indicated parameters in WT (n = 5) and Mst1^i∆EC (n = 5) mice. Data represent mean (bar) ± s.d. (error bars). P values, versus WT by two-tailed unpaired t-test. NS not significant. Source data are provided as a Source Data file