Fig. 8.

MST1–FOXO1 cascade is required for pathologic angiogenesis. a Images of CD31⁺ vessels in the superficial layer of retinas and avascular area (red) in WT-OIR, Mst1^i∆EC-OIR, and Foxo1^i∆EC-OIR mice. Scale bars, 500 μm. b Images of subcellular localization of FOXO1 in CD31⁺ vessels at vascular front (revascularization) and vascular plexus (neovascularization) in WT-OIR, Mst1^i∆EC-OIR, and Foxo1^i∆EC-OIR mice. Scale bars, 100 μm. Note that WT-OIR mice exhibited a nuclear localization of FOXO1 (yellow arrowheads), while Mst1^i∆EC-OIR mice showed a diffuse nucleocytoplasmic localization of FOXO1 (yellow arrows) in tip ECs and NVT ECs. c Comparisons of indicated parameters in WT-OIR (n = 5), Mst1^i∆EC-OIR (n = 5) and Foxo1^i∆EC-OIR (n = 5) mice. Data represent mean (bar) ± s.d. (error bars). P values, versus WT by two-tailed unpaired t-test. d Images of CD31⁺ vessels, ERG⁺ nuclei of ECs and GM130⁺ Golgi apparatus at tip ECs in WT-OIR, Mst1^i∆EC-OIR and Foxo1^i∆EC-OIR mice. The images of the inset (white dashed-line boxed) are magnified in e. The yellow dashed line outlines CD31⁺ vessels. Scale bars, 50 μm. e Images of ERG⁺ nuclei of ECs and GM130⁺ Golgi apparatus at tip ECs in WT-OIR, Mst1^i∆EC-OIR, and Foxo1^i∆EC-OIR mice. The yellow dashed line outlines CD31⁺ vessels. Note that GM130⁺ Golgi apparatus are polarized towards the anterior or posterior of the nuclei in tip ECs of WT-OIR mice (yellow arrowheads), while such polarization is lost in tip ECs of Mst1^i∆EC-OIR and Foxo1^i∆EC-OIR mice (yellow arrows). Scale bars, 100 μm. Source data are provided as a Source Data file