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. 2019 Feb 19;9:2234. doi: 10.1038/s41598-019-38913-z

Figure 5.

Figure 5

Comparison of yeast codon-optimized FPs (yFPs) versus the conventional FPs. (A) Comparison of FP brightness. Dots represent FP brightness of an individual cell, boxes indicate median with quartiles, whiskers indicate the 0.05–0.95 fraction of the datapoints. (B) Comparison of photostability. Dots represent FP photostability of an individual cell, boxes indicate median with quartiles, whiskers indicate the 0.05–0.95 fraction of the datapoints. (C) Comparison of FP expression. Dots represent fluorescence of the reference FP of an individual cell (divided by the total amount of exposure), boxes indicate median with quartiles, whiskers indicate the 0.05–0.95 fraction of the datapoints. (D) Overview of both the photostability and brightness of the yFPs and their FP counterparts. (E) pH sensitivity of the yFPs compared to the FPs. Solid lines with circles show yFPs fit and the data points, respectively. Dashed lines with rhombic points show FPs fit and the data points, respectively.