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. 2019 Feb 19;9:2234. doi: 10.1038/s41598-019-38913-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Monomerism of the yFPs. Cells expressing Cyterm-(y)FP were grown overnight at 30 °C and incubated for at least 1 hour at room temperature before microscope visualization. (A) Example of cells showing OSER structures and the pipeline to count OSER structures. (B) Monomerism of various FPs depicted by the fraction of cells with an OSER structure (left graph) and amount of OSER structures per cell (right graph). Error bars indicate 95CI. (C) Examples of the OSER assays per FP (max intensity projection of the Z-stack is shown).