Table. 1.

Peptide analysis with different types of FraC nanopores at pH 4.5

Peptide	Sequence	Molecular weight (g/mol)	Volume (nm³)	Charge		Iex% pH 4.5	Dwell time (ms)
				pH 4.5	pH 3.8
Wt-FraC type I pore, –30 mV
Endothelin 2	CSCSSWLDKECVYFCHLDIIW	2546.9	3.087	0.36	1.56	93.9 ± 1.8	104.0 ± 29.9
Endothelin 1	CSCSSLMDKECVYFCHLDIIW	2491.9	3.020	0.36	1.56	92.5 ± 0.5	19.73 ± 1.95
Dynorphin A	YGGFLRRIRPKLKWDNQ	2147.5	2.599	4.48	4.97	84.9 ± 2.6	3.68 ± 0.76
Pre-angiotensinogen 1–14	DRVYIHPFHLVIHN	1758.9	2.130	3.45	3.96	75.4 ± 2.3	0.29 ± 0.04
Angiotensin I	DRVYIHPFHL	1296.5	1.568	2.46	2.96	56.6 ± 0.9	0.15 ± 0.04
W116S-FraC type II pore, –30 mV
Angiotensin I	DRVYIHPFHL	1296.5	1.568	2.46	2.96	91.2 ± 0.2	0.54 ± 0.01
c-Myc 410-419	EQKLISEEDL	1203.3	1.456	−1.19	0.36	70.0 ± 3.4	0.12 ± 0.01
Angiotensin 1–9	DRVYIHPFH	1183.3	1.431	2.46	2.96	86.0 ± 0.2	0.37 ± 0.04
NLP-3 (66–75)	YFDSLAGQSL	1099.2	1.331	−0.52	0.97	75.3 ± 3.0	0.23 ± 0.02
Angiotensin II	DRVYIHPF	1046.2	1.266	1.47	1.96	82.1 ± 1.3	0.37 ± 0.04
Asn1Val5 AngioII	NRVYVHPF	1031.2	1.248	2.03	2.16	80.4 ± 0.2	0.34 ± 0.06
Angiotensin A	ARVYIHPF	1002.2	1.212	2.03	2.16	79.0 ± 0.6	0.34 ± 0.02
Angiotensin III	RVYIHPF	931.1	1.127	2.03	2.16	77.9 ± 0.5	0.35 ± 0.04
Ile7 Angiotensin III	RVYIHPI	897.1	1.085	2.03	2.16	75.7 ± 0.4	0.19 ± 0.05
Angiotensin IV	VYIHPF	774.9	0.938	1.02	1.16	61.1 ± 4.0	0.15 ± 0.06
W112S-W116S-FraC type III pore, –50 mV
Angiotensin IV	VYIHPF	774.9	0.938	1.02	1.16	98.9 ± 0.8	0.61 ± 0.07
Angiotensin 4–8	YIHPF	675.8	0.818	1.02	1.16	91.8 ± 0.4	0.40 ± 0.04
Endomorphin I	YPWF	610.7	0.741	0.04	0.17	80.3 ± 0.5	0.32 ± 0.04
Met5 Enkephalin	YGGFM	573.7	0.695	0.04	0.17	66.5 ± 0.7	0.16 ± 0.02
Leucine Enkephalin	YGGFL	555.6	0.673	0.04	0.17	65.6 ± 2.4	0.20 ± 0.05

The charges of the peptides were calculated according to the pK_a for individual amino acid⁵³. Standard deviations were obtained for at least three measurements

FraC fragaceatoxin C, Wt-FraC wild-type FraC