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. 2019 Feb 19;9:2262. doi: 10.1038/s41598-018-38458-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Improved miRNA capture also seen at low concentrations of input RNA. (A) The percentage of reads mapping to miRNAs at the indicated input following either the optimized (0.05 μΜ 3′ adapter, 0.33 μΜ amino-modified 5′adapter) or the original (0.85 μM 3′ adapter, 3.3 μM unmodified 5′ adapter) protocol. Black dots represent the three replicates from each input RNA for each sample, protocol, and concentration. Red dots represent average. (B) The average percentage of reads mapping to different RNA classes for each sample, protocol, and concentration.