Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 19;9:2314. doi: 10.1038/s41598-018-38294-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Chrnb4-GFP and Chrnb4-cre expression in developing and adult cone photoreceptor cells. (A) Immunostaining of adult (6 week old) Chrnb4-GFP retinas. Chrnb4-GFP expression co-labels with the expression of cone marker CA in the ONL. (B–D) Immunostaining of P8 Chrnb4-GFP retinas. Chrnb4-GFP expression co-labels with the expression of cone markers RxRγ (B) and CA (C) but not with the expression of rod marker RHO (D). See Supplementary Figs 3 and 4 for the separate channels of the data shown in the merged images in A & B. (E,F) Immunostaining on E17 Chrnb4-cre; R26^YFP retinas using an anti-GFP antibody to amplify the YFP signal intensity. YFP expression is detected in some RxRγ^+ve cones (white arrows), while most cones remain YFP^-ve (E, yellow arrows). YFP expression is also seen in some OTX2-expressing photoreceptor progenitors (F, white arrows). Yellow arrows indicate OTX2^+ve cells that are not YFP^+ve. OTX2^+ve RPE cells do not express YFP (blue arrows). (G–I) Immunostaining on adult (6 weeks old) Chrnb4-cre; R26^YFP retinas. YFP expression was detected in CA^+ve cones (G, white arrows) and in a few inner retinal cells including some PAX6^+ve cells (H, white arrowheads). Most Pax6^+ve cells in the inner retina remained YFP^-ve (H, yellow arrowheads). CHX10^+ve bipolar cells were mainly YFP^-ve (I, asterisks). (J) Immunostaining on E12 Chrnb4-cre; R26^YFP retinas was also performed. YFP expression was detected in a subset of CHX10^+ve RPCs (white arrows). CA: cone arrestin. RxRγ: retinoid x receptor gamma. RHO: rhodopsin. RPE: retinal pigment epithelium. ONL: outer nuclear layer. INL: inner nuclear layer. GCL: ganglion cell layer. RPC: retinal progenitor cells. Scale bars: 30 μm. Scale bars of insets: 10 μm.