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. 2019 Feb 19;9:2259. doi: 10.1038/s41598-019-38674-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Preparation of adsorbed (AM) (a,b) and spread (SM) (c) monolayers at the O/W interface. The lipid solution was deposited over either the upper phase (a) or at the O/W interface after preparing the liquid-liquid biphasic system (b) or was spread onto the A/W interface before pouring the oil to obtain the O/W interface (c). Insets: (a) molecules of phospholipid diffuse radially both in the volume of the oil phase and along the O/W interface; (b,c) radial diffusion occurs bi-dimensionally along the O/W or the A/W interfaces, respectively.