Table 3.
Mutation rates (%) at positions 67 and 71 of the P35S sequence using raw reads and corrected consensus(3) or consensus(10) reads.
| Position 67 | Mutation A → T | Other mutations | ||
|---|---|---|---|---|
| 10% C4 | 1% C4 | 0.1% C4 | mean | |
| Raw reads | 9.0386 | 0.9705 | 0.0599 | 0.1984 |
| Consensus (3) reads | 1.4797 | 0.1426 | 0.0065 | 0.0413 |
| Consensus (10) reads | 0.3555 | 0.0226 | 0.0000 | 0.0175 |
| Position 71 | Mutation A → G | Other mutations | ||
| 10% C1 | 1% C1 | 0.1% C1 | mean | |
| Raw reads | 10.6484 | 0.5923 | 0.0649 | 0.1626 |
| Consensus (3) reads | 1.9858 | 0.1066 | 0.0080 | 0.0318 |
| Consensus (10) reads | 0.4841 | 0.0308 | 0.0000 | 0.0158 |