TABLE 1.

Summary of sequencing results for vaginal samples^a

Library preparation method parameter	Result by PCR type
	1-step	2-step	2-step
No. of samples subjected to amplification	92	92	92
No. of samples successfully amplified	49	83	83
Sequencing platform	MiSeq	MiSeq	HiSeq
Chimeric sequences detected (%)	0.70	3.3	3.1
Mean no. (±SE) of nonchimeric, assembled sequences per sample	11,080 ± 1,506	14,282 ± 483	50,514 ± 4,427
Median quality score per sample (Q1–Q3)	36.2b (33.5–37.2)	34.9b (29.9–36.3)	37.1b (33.0–38.0)

^aFigure S4 summarizes the prequality filtering per-cycle quality scores.

^bSignificant. Kruskal-Wallis, H = 187.85; P < 2.2 × 10⁻¹⁶.