Table 1.
Primary and secondary antibodies used
| Host | Vendor | Catalog no. (RRID no.) | Concentration | |
|---|---|---|---|---|
| Primary antibodies | ||||
| Anti–βIII-tubulin | Rabbit | Covance | PRB-435P-100 (AB_291637) | 1:1000 |
| Anti-CGRP | Rabbit | Peninsula | T-4032 (AB_2313775) | 1:1000 |
| Anti-GFP | Rabbit | Invitrogen | A-11122 (AB_221569) | 1:1000 |
| Anti-βgal | Rabbit | MP Biochemicals | ab6645 (AB_2313831) | 1:500 |
| IB4 | Sigma | L2140 (AB_2313663) | 1:100 | |
| Anti-NF200 | Chicken | Aves Labs | NF-H (AB_2313552) | 1:1000 |
| Anti-Tag-1 | Goat | R&D Systems | AF1714 (AB_2245173) | 1:1000 |
| Secondary antibodies | ||||
| Anti-rabbit DyLight 488 | Donkey | Jackson ImmunoResearch Laboratories | 711-485-152 (AB_2492289) | 1:500 |
| Anti-rabbit Cy-3 | Donkey | Jackson ImmunoResearch Laboratories | 711-166-152 (AB_2313568) | 1:500 |
| Anti-rabbit Cy-5 | Donkey | Jackson ImmunoResearch Laboratories | 711-175-152 (AB_2340607) | 1:500 |
| Anti-rabbit Alexa Fluor 488 | Donkey | Invitrogen | A-21206 (AB_141708) | 1:500 |
| Anti-rabbit Alexa Fluor 594 | Donkey | Invitrogen | A-21207 (AB_141637) | 1:500 |
| Anti-rabbit Alexa Fluor 647 | Donkey | Invitrogen | A-31573 (AB_2536183) | 1:500 |
| Anti-goat Alexa Fluor 488 | Bovine | Jackson ImmunoResearch Laboratories | 805-545-180 (AB_2340883) | 1:500 |
| Anti-goat Cy-3 | Bovine | Jackson ImmunoResearch Laboratories | 805-165-180 (AB_2340880) | 1:500 |
| Anti-goat Alexa Fluor 647 | Bovine | Jackson ImmunoResearch Laboratories | 805-605-180 (AB_2340885) | 1:500 |
| Anti-chicken Alexa Fluor 488 | Donkey | Jackson ImmunoResearch Laboratories | 703-545-155 (AB_2340375) | 1:500 |
| Anti-chicken Cy-3 | Donkey | Jackson ImmunoResearch Laboratories | 703-165-155 (AB_2340363) | 1:500 |
| Anti-chicken Cy-5 | Donkey | Jackson ImmunoResearch Laboratories | 703-175-155 (AB_2340365) | 1:500 |
| Alexa 488-conjugated Streptavidin | Thermo Fisher Scientific | S11223 (AB_2336881) | 1:500 | |