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RSD caused mechanical allodynia in an exposure-dependent manner. Male C57BL/6 mice were subjected to 6 d of RSD (Stress) or left undisturbed as controls (Control). Mechanical allodynia was assessed before stress exposure and 12 h after the first, third, and sixth day of RSD. Withdrawal threshold of mechanical stimulation to the hindpaw using the von Frey behavior test was determined. Error bars indicate mean ± SEM. *Significantly different from control mice (p < 0.05).
