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. 2019 Feb 13;10:154. doi: 10.3389/fmicb.2019.00154

FIGURE 1.

FIGURE 1

Organization of rRNA operons on NGR234 chromosome and detailed genetic maps of the rRNA1 and rRNA3 loci. (A) Circular map of the 3.9 Mb chromosome of NGR234 with the positions and orientations of the three rRNA operons as reported in the accession number NC_012587 (Schmeisser et al., 2009). (B) Detailed genetic maps of the rRNA1 and rRNA3 operons: Genes coding for 5S, 16S, and 23S rRNA subunits are shown as yellow arrows oriented according to transcription, while genes predicted to code for proteins are shaded in gray and those for tRNAs depicted as green triangles. The section of each operon that was replaced by Omega is shaded in gray. Segments of the 16S genes, promoters, and terminators that were verified by sequencing cosmid DNAs are highlighted in darker shades of gray. Regions flanking the deleted rRNA operons and used as sites for homologous recombination are delimited by dashed lines. Small and labeled white arrows represent the positions and orientations of primers used in this work.