Fig. 4.

Association of PrP^TSE and p.tau in the 87 V/VM and ME7/C57BL scrapie mouse models.

Confocal analysis of plaque like PrP^TSE (red) and p.tau (green) in the cortex of 87V infected VM mice (a) and in the septum of ME7 infected C57BL mice (b). 3D-Imaris rendering revealing close association and interaction of PrP^TSE and p.tau (c,d). Images obtained after double labelling with anti-PrP antibody 1B3 and anti-p.tau antibody AT8 and Alexa 488 and 594 fluorescent secondary antibodies. Scale bar = 5 μm (a and c), scale bar 10 μm (b) corresponding to panels b and d. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)