Fig. 5.

Abundant PrP with minimal p.tau accumulation in GSS-22 mice.

Severe spongiform encephalopathy (a, d) and numerous plaques, (arrows in d) in the cerebral cortex of GSS-22 mice overexpressing PrP 101L. Numerous PrP deposits in the cerebral cortex (b, e, g) and hippocampus of GSS-22 mice (b). Minimal p.tau positivity in the cortex (c, f, and h (arrow)). Light microscopy images obtained after staining with haematoxylin and eosin (a, d), anti-PrP antibody 6H4 (b, e) and anti-p.tau antibody AT8 (c, f) and counterstained with haematoxylin. Confocal images obtained after double labelling with anti-PrP antibody 1B3 and anti-p.tau antibody AT8 and Alexa 488 and 594 fluorescent secondary antibodies. Scale bar = 500 μm (a–c); scale bar = 100 μm (d–f), scale bar = 20 μm (g), scale bar = 10 μm (h).