Fig. 5.

UCA1 siRNA suppressed the expression of the downstream key effectors in CAF-CM treated SW480 cells. a SW480 cells were cultured in normal DMEM (as control), CAF-CM, CAF-CM + NC siRNA and CAF-CM + UCA1 siRNA for 48 h then UCA1 mRNA level evaluated by qRT-PCR. b Effects of UCA1-siRNA and NC siRNA transfection on mRNA level of downstream key molecules. CAF-CM-treated SW480 cells were transfected with UCA1-siRNA or NC siRNA and transcript levels of mTOR, cyclin D1, p27, miR-143 and KRAS was evaluated by qRT-PCR. c CAF-CM-treated SW480 cells were transfected with UCA1-siRNA or NC siRNA and the protein levels of mTOR, cyclin D1, p27, P-p27 and KRAS were detected by western blot analysis. d, e Transfected SW480 cells cultured in CAF-CM and transwell assay was performed to determine the suppression effects of UCA1-siRNA on migration and invasion in CRC cell after 48 h. UCA1 knockdown significantly decreased the migration and invasion in CRC cell. f UCA1 knockdown significantly inhibited the cell proliferation of SW480 cells compared with NC siRNA group after 24 h and 48 h, as measured by MTT assay. *: P < 0.05; **: P < 0.01; ***: P < 0.001