Fig. 4.

Proliferation retardation of primitive leukemic hematopoietic progenitor colonies by anti-Wnt3a antibody and N-Cadherin expression in the leukemic marrow. a Representative CFU-GEMM, CFU-GM, CFU-G and BFU-E colonies in methylcellulose based semi-solid media [Magnification 400X]. b Bar diagrams represented the number of control and leukemic hematopoietic progenitor colonies with and without anti-Wnt3a antibody. The numbers of leukemic CFU-GEMM and BFU-E colonies were decreased significantly after anti-Wnt3a antibody supplementation. c Histogram overlay plot and bar diagram showed significant up-regulation of N-Cadherin in the leukemic HSPC compartment. d Representative immunofluorescence images showed N-Cadherin expression in the control and leukemic marrow cells. N-cadherin expression was higher in the leukemic marrow cells (lower panel) [Scale bar 10.0 μm]