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. 2018 Jul 5;13(1):39–52. doi: 10.1007/s12079-018-0470-6

Fig. 5.

Fig. 5

Malignant transformation alters BM hematopoietic stromal microenvironment and stroma derived Wnt. a Representative images of dayswise LT-BMC of control and leukemic mice recorded at different time points, viz day 7, 10, 15 and 20. Left panel (inside black border): control BM derived stromal cell generation and their gradual proliferation pattern. Right panel (inside red border): Leukemic BM derived stromal cell generation and their proliferation pattern. Early generation and rapid transformation of stromal precursors to stromal fibroblasts and their higher proliferation potential in leukemic condition [orange arrow: stromal fibroblasts; black arrow: stromal precursors]. b-c Representative ICC images. Wnt3a expression in Bi. Control and Bii. Leukemic BM derived stromal cells. Leukemic stromal cells showed higher Wnt3a protein expression. Wnt5a expression in Ci. Control and Cii. Leukemic BM derived stromal cells. Leukemic stromal cells showed lower Wnt5a protein expression. The brown depositions were the reaction product of substrate DAB and HRP tagged with secondary antibody and its intensity was directly proportional to the protein expression level [Magnification 1000X]. d. Comparative dot blot assay showed decreased level of Dkk1 in the leukemic stromal cell culture supernatant. [red arrow: control; blue arrow: leukemia]