Fig. 3.

The bindings of FLAG-tagged rTSK to four domains of CCN2 (IGFBP, VWC, TSP1, or CT) or none were measured with solid-phase binding assay by using anti-FLAG mouse Ab and anti-mouse IgG-HRP as described in Materials and methods. The absorbance values at 450 nm indicates the amount of HRP bound to wells coated each domain of CCN2 or none. Statistical analysis was performed by performing Dunnett’s t-test and the One-way ANOVA test, *p < 0.001, as compared with the values for wells with 0 μg/ml rTSK. Data were expressed as the means of three independent wells ± standard deviation (SD)