Fig. 3.

HepG2 and SMMC-7721 cells were transfected with the pcDNA-NRAL plasmid and its negative control, while HepG2/CDDP and SMMC-7721/CDDP cells were transfected with pshR1+3-NRAL and its negative control. pcDNA-NRAL plus miR-340-5p-expressing plasmids or pshR-NRAL plus ASO miR-340-5p were used for rescue experiments in both parental and resistant cells. (a) Transfected cells were seeded into a 96-well plate at a density of 5×10³ cells per well in triplicate. After 8 hrs, the cells were treated with the indicated doses of CDDP for 48 hrs. Then, the cells were harvested for apoptosis analysis by double annexin-V/PI staining and flow cytometry. *p < 0.05, **p < 0.01 vs the NC group, ^#p < 0.05. (b) A caspase-Glo3/7 assay kit (Promega) was used for detecting cleaved caspase3 activity. *p < 0.05, **p < 0.01 vs the NC group, ^#p < 0.05