Fig. 5.

NRAL controls the target of miR-340-5p. (a) Schematic diagram of the miR-340-5p binding site in the Nrf2 3’UTR or its mutant sequences based on TargetScan software. (b) Relative luciferase intensities of the luciferase constructors harbouring the wild-type or mutant Nrf2 3’UTRs were measured in 293T cells 48 h after transfection with the pri-miR-340-5p plasmid or ASO-miR-340-5p, n=3, **p < 0.01 vs the other group. (c) pmirGLO-Nrf2-3’-UTR and pri-miR-340-5p plasmids were co-transfected into HEK293T cells with plasmids expressing NRAL (pcDNA/NRAL) or NRAL- mut (pcDNA/NRAL-mut) with a control vector to verify the ceRNA activity of NRAL. miRNA-141 was used as a negative control. The histogram indicates the luciferase values 48 h after transfection