Fig. 6.

(a-b) mRNA (A, *p < 0.05 vs the NC group, **p < 0.01, ***p < 0.001 vs the pcDNA group) and protein (b) levels of Nrf2 and Keap1 when the NRAL expression level was altered. β-actin was used as a reference gene, and the normalized values represent the means from at least 3 separate experiments, *p < 0.05 vs the pcDNA group, **p < 0.01 vs the NC group. (c-d) miR-340-5p-expressing plasmids cause a dose-dependent decrease in the protein expression of Nrf2. pcDNA/NRAL caused a dose-dependent increase in the protein expression of Nrf2. β-actin was used as a reference gene, and the normalized values represent the means from at least 3 separate experiments, *p < 0.05 vs the miR NC group,^#p < 0.05 vs the 30 nm group, ^&p < 0.05 vs the 50 nm group