Figure 5.

FGF‐18 promotes early chondrogenesis and maturational osteogenic differentiation of ovine stromal stem cells grown in micromass pellet culture. (D), (E): Decorin and biglycan were strongly upregulated by FGF‐18 on days 31–41. The CS sulfation motifs 4‐C‐3 and 7‐D‐4 (G), (H) followed a similar deposition to that of calcium in the pellets evident by Alizaran staining (I). Figure modified from 40 with permission. Histograms depict RTPCR data showing the increase in decorin and biglycan expression with FGF‐18 treatment (J), and densitometric morphological data depicting Calcium deposition levels in pellets (K). Immunolocalization of 3‐B‐3(−), 7‐D‐4, and 4‐C‐3 CS sulfation motifs in transitional tissues in fetal knee joint development in a 14 –week‐old gestational age human fetal knee. 3‐B‐3(−), 7‐D‐4, and 4‐C‐3 were immunolocalized in the rudiment tibial surface, perichondrium, and tibial growth plate. Figure modified from 41 with permission.