Fig. 4.

The arrest/lethal phenotype resulting from GFP-fused D282A mutant Strongyloides stercoralis (Ss)-RIOK-1 expression was rescued by co-expression of GFP-fused wild-type Ss-RIOK-1. Percentages of larvae classified into three developmental classes, young larvae, moulting larvae and infective L3s (iL3), from groups transformed with plasmid mixtures containing varying ratios of the mutant construct (pRP8) to the wild-type construct (pRP7), with the mutant or wild-type construct alone and the non-injection group. All the transgenic larvae were collected and classified into the three groups, including dead worms. Counts were done at 96 h after transformation. Error bars indicate S.D. Asterisks denote the proportion of iL3s in these groups were statistically much higher than that in pRP8 group, which was revealed by one-way ANOVA analysis with Bonferroni’s Multiple Comparison Test. *P < 0.05, ^**P < 0.01, ^***P < 0.001.