Figure 4.

MAP7D2 Interacts with Kinesin-1 Family and Affects Kinesin-1 Distribution in the Proximal Axon

(A) Indicated baits incubated with rat brain and HEK293 cell extracts identified proteins by mass spectrometry. PD-MS means pull-down-based mass spectrometry.

(B) Western blot analysis of biotinylated (bio) pull-down from extracts of HEK293 cells transfected with indicated constructs, probed for GFP and mCherry.

(C) Representative images of neurons with control, MAP7D2 sh1, or sh3 and fixed at DIV3 and stained for KIF5C and the axonal marker Tau. Arrowheads point to the proximal axon.

(D and E) Quantifications of KIF5C intensity (D) or ratio (E) at the beginning and the end of axon in indicated conditions. n = 15–32 neurons.

(F and G) Quantifications of KIF5C intensity (F) or ratio (G) at the beginning and the end of axon in specified conditions. n = 19–27 neurons.

^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001. Unpaired t test. Error bars represent SEM. Scale bars: 20 μm in (C).