Figure 2.

Fragmentation of Fibrils in the Absence of Soluble Tau Proteins

(A and B) Aggregated tau samples were centrifuged. The pellet was resuspended in fresh proteasome buffer followed by incubation with (A) buffer control or (B) the proteasome holoenzyme for 20 h and subsequently imaged and presented as described in Figure 1.

(C and D) Proteasome holoenzymes pre-treated with (C) Velcade (proteasome^Velcade) or (D) ATPγS (proteasome^ATPγS) were subsequently incubated with aggregated tau as in (B).

(E) Instead of the holoenzyme, fibrils were also incubated with regulatory particles (RPs) and analyzed as above.

Combined results of three independent experiments (n = 3) are shown.