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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Neurobiol Dis. 2018 Nov 10;124:141–151. doi: 10.1016/j.nbd.2018.11.005

Figure 8. Microglia Activation After SRS.

Figure 8.

Brain sections (15 μm) from a 1-in-10 series were selected. (A, D) Sections were stained with Iba1 antibodies to detect reactive microglia in CA1 and CA3 regions of hippocampus. Scale bar represents 100 μm. (B, E). The levels of Iba1+ cells were obtained by counting the three sections and averaged to obtain a representative number of Iba1+ cells in a particular animal. Cell counts were conducted blinded to seizure frequency. Data is presented as the mean ± SEM of controls (n=3) and animals that experienced SRS (n=8). A significant difference in the number of Iba1+ cells in controls and animals experiencing SRS was detected using an unpaired t-test, *p<0.05 and **p<0.01 represent a significant difference. (C, F) A linear regression analysis between the number of Iba1+ cells and the number of SRS did not show a significant correlation when all animals are included (blue line). When an animal experiencing the highest number of seizures (blue dot) was not part of the analysis (black dots and line), the plots displayed a significant correlation in CA1 (R2=0.7814, p=0.0007) and CA3 (R2=0.7645, p=0.0009) regions.