Fig. 3.

The absence of IFT140 affects the abundance and localization of IFT-A but not of IFT-B. (A) Western blot showing that loss of IFT140 in ift140-1 cells severely affected the levels of the IFT-A proteins IFT139 and IFT122. In contrast, levels of the IFT-B proteins examined remained unchanged. βF1 ATPase is the loading control. (B) SIM images of IFT components in wild-type and ift140-1 cells. In both cell types, IFT-B – as represented by IFT57 and IFT172 – localized in ring-shaped structures near the basal body. This suggests that IFT-B can localize properly in the peri-basal body area in the absence of IFT-A. IFT139 was not detected in ift140-1 cells by SIM, consistent with western blot results.