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. 2019 Feb 11;132(3):jcs220749. doi: 10.1242/jcs.220749

Fig. 8.

Fig. 8.

Effect of IFT140 WD repeat loss on the protein composition of flagella. (A) SDS-PAGE of axoneme and ‘membrane+matrix’ fractions from IFT140-G/IFT20-mC and IFT140ΔWD-G/IFT20-mC flagella. Asterisks indicate differences between bands in the IFT140-G/IFT20-mC and IFT140ΔWD-G/IFT20-mC ‘membrane+matrix’ fractions. (B) Proteins identified by MS of IFT140-G/IFT20-mC and IFT140ΔWD-G/IFT20-mC ‘membrane+matrix’ fractions were curated into separate groups and individual fold-change values (IFT140ΔWD-G/IFT20-mC:IFT140-G/IFT20-mC) based on iBAQ scores are shown in the graph. Red lines indicate a 2.0-fold increase and a 2.0-fold decrease. Green lines indicate median values for each protein group. IFT-A, GTPases, myristoylated proteins and geranylgeranylated proteins all have their median values decreased ≥2.0-fold, suggesting that recruitment of these protein classes is impaired in IFT140ΔWD-G/IFT20-mC flagella. The proteins included in each class are listed in Tables S1–S11.