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. Author manuscript; available in PMC: 2019 Feb 20.
Published in final edited form as: Curr Opin Biotechnol. 2018 Oct 13;55:151–158. doi: 10.1016/j.copbio.2018.09.006

Figure 2.

Figure 2

Affinity-based methods for optical detection of epigenetic marks. (a) Single-molecule detection of DNA methylation sites. Methylation sites on stained DNA are labeled with fluorescent MBD1 protein. Two-color labeled DNA is then loaded on a nano-fluidic device, detected and sorted according to its fluorescent signature [15]. (b) Single-molecule detection of post translational modifications on nucleosomes. Nucleosomes are prepared by MNase digestion. DNA ends are ligated to fluorescent biotinylated adaptors, purified and captured on PEG-streptavidin-coated slides. Attached nucleosomes are incubated with fluorescently-labeled antibodies to histone modifications which can be then imaged. Finally, histones are removed, and their genomic position is determined by single-molecule sequencing-by-synthesis [18]. Reprinted with permission from AAAS.