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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Ann Surg. 2018 Oct;268(4):564–573. doi: 10.1097/SLA.0000000000002977

FIGURE 2.

FIGURE 2.

Rhesus Macaque IgG and IgM antibody binding to pig PBMC. (A) Flow cytometric histograms showing IgG, top row, and IgM, bottom row, binding to three genetically modified pig backgrounds. Each representative histogram is overlaid on secondary alone in gray. (B) Flow cytometric MFI of IgG and IgM antibody binding of a group of normal Rhesus Macaque animals (n=14) is shown along X axis and Y axis respectively. GGTA1, GGTA1/B4GalNT2, and lastly GGTA1/CMAH/B4GalNT PBMC antibody binding is demonstrated. Box in left lower corner demonstrates region of estimated negative crossmatch.