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. Author manuscript; available in PMC: 2020 Jan 3.
Published in final edited form as: Mol Cell. 2018 Nov 29;73(1):36–47.e10. doi: 10.1016/j.molcel.2018.10.032
TMT mix knocked out gene(s) strain TMT label

80S-1 Wild-type 4484 126C
80S-1 RPL41B 7461 127N
80S-1 RPL26A 3851 128N
80S-1 RPL26B 2374 128C
80S-1 RPL26A and RPL26B 5048 129N
80S-1 RPL40B 2700 130N
80S-1 RPL40A 7889 130C
80S-1 RPL24A 7469 131N
80S-1 RPL7A 7962 131C

80S-2 wild-type 4484 126C
80S-2 RPL41A 7467 127N
80S-2 wild-type 4484 127C
80S-2 RPS22B 4662 128N
80S-2 RPS28A 4472 128C
80S-2 RPS25B 4658 129N
80S-2 RPS25A and RPS25B 7463 129C
80S-2 RPS22A het and RPS22B het 4665 130N
80S-2 RPS29B 4480 130C
80S-2 RPS22A 4659 131N
80S-2 RPS28B 4474 131C

60S RPL26A and RPL26B (60S full
region)
5048 127N
60S RPL40B (60S full region) 2700 127C
60S RPL7A (60S full region) 7962 128N
60S WT (60S full region) 4484 129N
60S RPS28A (60S full region) 4472 129C
60S RPS29B (60S full region) 4480 131N
60S RPS22A (60S full region) 4659 131C

TMT mix knocked out gene(s) strain TMT label

80S-1 Wild-type 4484 126C
80S-1 RPL41B 7461 127N
80S-1 Wild-type 2 1362 127C
80S-1 RPL26A 3851 128N
80S-1 RPL26B 2374 128C
80S-1 RPL26A and RPL26B 5048 129N
80S-1 RPL24B 7465 129C
80S-1 RPL40B 2700 130N
80S-1 RPL40A 7889 130C
80S-1 RPL24A 7469 131N
80S-1 RPL7A 7962 131C

80S-2 wild-type 4484 126C
80S-2 RPL41A 7467 127N
80S-2 wild-type 4484 127C
80S-2 RPS22B 4662 128N
80S-2 RPL28A 4472 128C
80S-2 RPS25B 4658 129N
80S-2 RPS25A and RPS25B 7463 129C
80S-2 RPS22A het and RPS22B het 4665 130N
80S-2 RPS29B 4480 130C
80S-2 RPS22A 4659 131N
80S-2 RPS28B 4474 131C

60S RPL26A (60S full region) 3851 126C
60S RPL26A and RPL26B (60S full
region)
5048 127N
60S RPL40B (60S full region) 2700 127C
60S RPL7A (60S full region) 7962 128N
60S empty empty 128C
60S WT (60S full region) 4484 129N
60S RPS28A (60S full region) 4472 129C
60S RPS29A (60S full region) 4479 130N
60S RPS29B (extra 60S alone) 4480 130C
60S RPS29B (60S full region) 4480 131N
60S RPS22A (60S full region) 4659 131C