Table 2. Nucleotide polymorphisms in 12 TULV genomes from the common vole hybrid zone between the Central and Eastern evolutionary lineage.
| TULV-CEN.S (n = 6) | TULV-EST.S (n = 6) | Between clades (n = 12) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Length CDS | Pn | Ps | dN/dS | Pn | Ps | dN/dS | Dn | Ds | dN/dS | |
| S-segment | 1,287 nts | 2 | 176 | 0.003 | 3 | 156 | 0.006 | 6 | 42 | 0.007 |
| M-segment | 3,423 nts | 33 | 728 | 0.008 | 9 | 408 | 0.006 | 5 | 227 | 0.004 |
| L-segment | 6,459 nts | 58 | 1,193 | 0.008 | 24 | 1,076 | 0.006 | 29 | 270 | 0.007 |
| Concatenated segments | 11,169 nts | 93 | 2,097 | 0.009 | 36 | 1,640 | 0.006 | 40 | 539 | 0.007 |
Gene CDSs of the small, medium, and large TULV genome segments were analyzed separately and as concatenated sequences. The number of polymorphic nonsynonymous and synonymous positions within the TULV-CEN.S and TULV-EST.S virus clades are indicated. Nucleotide divergence between TULV clades is quantified as nonsynonymous and synonymous positions with variants private to one viral clade. The ratio of nonsynonymous to synonymous substitutions is given for individual clades and comparing all genomes.
Abbreviations: CDS, coding sequence; Dn, number of nonsynonymous positions with variants private to one viral clade; DS, number of synonymous positions with variants private to one viral clade; dN/dS, ratio of nonsynonymous to synonymous substitutions; L-segment, large genome segment; M-segment, medium genome segment; nts, nucleotides; Pn, number of polymorphic nonsynonymous positions; PS, number of polymorphic synonymous positions; S-segment, small genome segment; TULV, Tula orthohantavirus; TULV-CEN.S, Central South TULV; TULV-EST.S, Eastern South TULV.