Skip to main content
. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Neurobiol Dis. 2018 Dec 19;124:379–395. doi: 10.1016/j.nbd.2018.12.007

Fig. 3.

Fig. 3.

Upregulation of TFEB by cinnamic acid. (A-D) Cinnamic acid transcriptionally upregulate Tfeb. (A) Design of pTFEB(WT)-luciferase or pTFEB(Mutant)-luciferase construct. (B) WT astrocytes were transfected with pTFEB(WT)-luciferase or pTFEB(Mutant)-luciferase for 24 h followed by cinnamic acid treatment (50, 100, 200uM) for 6 h and subsequently subjected to luciferase assay. (C-D) WT astrocytes were treated with 100 and 200uM of cinnamic acid for 2 h followed by chromatin immunoprecipitation to analyze the recruitment of PPARα, β, γ, RNA polymerase, CBP and p300 to the Tfeb promoter by (C) semi-quantitative RT PCR and (D) real time PCR. (E-H) Cinnamic acid increases TFEB expression in primary brain cells. Mouse primary astrocytes were treated with (E) different doses (50, 100, 200uM) of cinnamic acid for 8 h, (F) 100uM cinnamic acid for 2, 6, 12, 24 h followed by Tfeb mRNA expression analysis by real time PCR; (G-H) TFEB expression level was monitored in (G) primary astrocyte and (H) cultured cortical neurons treated with 100uM cinnamic acid for 24 h. Scale bar 10um. Data represents fold change mean ± SD with relative to the untreated control. Student’s paired t-test was used for statistical analysis. * p < .05; ** p < .01; *** p < .001.