Figure 6. Gliclazide (GLI) modulates macrophage migration inhibitory factor (MIF) expression. Representative confocal photomicrographs of MIF immunoreactivity in periodontal specimens from each group (green) with DAPI nuclear counterstaining (blue). (A) Sample from the no ligature group showing discrete MIF labeling in dentin (d), periodontium (p), or alveolar bone (ab); (B) Sample from the ligature group shows strong labeling (red arrows) in the periodontal area. (C) Sample from the 1 mg/kg GLI group showing diffuse, weak MIF labeling (red star). Scale bar 50 µm, 200× magnification. (D) Densitometry analysis confirmed significantly decreased MIF immunoreactivity in the 1 mg/kg GLI group, which was blocked in the ligature group. Five immunofluorescence sections from each animal (n=3/group) were analyzed. ***p<0.001, Kruskal–Wallis test followed by Dunn’s test.