Figure 7. Gliclazide (GLI) modulates NFKB P50 (Cytoplasm), NFKB P50 (Nuclear), AKT, PI3K immunoreactivity in periodontal specimens from each group (green) with DAPI nuclear counterstaining (blue). Densitometry analysis confirmed significantly increased NFKB p50 (cytoplasm) immunoreactivity relative NFKB p50 (Nuclear) in the L group (p<0.05). Representative confocal photomicrographs of PI3K immunoreactivity was indicated by strong labeling in the periodontal area of samples from the L group. Discrete Pi3K labeling was observed in alveolar bone in samples from the NL group and weak, diffuse labeling was observed in samples from the 1 mg/kg GLI group, p<0.05. Asterisks: Dental tissue; Arrows (red): Cells. Scale bar 50 µm, 200× magnification. Five immunofluorescence sections from each animal (n=3/group) were analyzed. ***p<0.001, Kruskal-Wallis test followed by Dunn’s test.