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. 2019 Jan 31;70(4):1267–1281. doi: 10.1093/jxb/ery458

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© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Anti-callose immunogold labeling of embryogenic microspores rapeseed line DH4079 and eggplant line DH36. (A) Rapeseed DH4079 embryogenic microspore and (B) detail of the inner cell wall (cw) and subintinal layer (sl) showing abundant gold particles. The asterisk indicates deposits of excreted material. (C) Eggplant DH36 embryogenic microspore, and detail of (D) the SL showing abundant gold particles and (E) the inner cell wall showing very scarce, dispersed gold particles. Ct, cytoplasm; ex, exine; in, intine. Scale bars: (A, C) 10 µm, (B, D, E) 500 nm.