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Microspores of rapeseed line DH4079 treated with 1 mg l–1 digitonin. Paired images are shown of staining with aniline blue (AB) for callose and propidium iodide (PI) for cytoplasm, and with S4B for cellulose (red) and DAPI. (A, A′) Embryogenic microspores at 3 d old. Note the presence of multiple ectopic, cytoplasmic wall fragments. (B, B′) Embryogenic microspores at 6 d old. ct, cytoplasm; cw, cell wall; ex, exine; n, nucleus; sl, subintinal layer. Scale bars are 10 µm.
