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. 2018 Dec 7;70(4):1167–1182. doi: 10.1093/jxb/ery429

Fig. 5.

Fig. 5.

Knock-out of SiSTL1 homologous gene in rice. (A) Phylogenetic tree of RNRLs in Arabidopsis, Oryza sativa, Zea mays, Sorghum bicolor, and Setaria italica based on protein sequences. Black arrow indicate the causal gene for sistl1. White arrows indicate two other genes encoding SiSTL1 homologues in Setaria (SiSTL1-2 and SiSTL1-3). The homolog of SiSTL1 in rice (OsRNRL1, LOC Os06g07210) is highlighted by shading. (B) Position of two CRISPR targets in OsRNRL1 and sequence variations in the striped transgenic T0 plants, OsC1-2, OsC1-4, OsC1-8, OsC2-12, and three other chimeric plants. (C, D) T1 plants of OsC1-8 grown in a paddy field. OsC1-8 T1-W, normal green individual; OsC1-8 T1-1, striped-leaf individual. (E, F) Resin sections of OsC1-8 T1-W (E) and striped OsC1-8 T1-1 (F) leaf fragments. (G, H) Ultrastructures of OsC1-8 T1-W (G) and striped OsC1-8 T1-1 (H) leaf fragments.