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. 2019 Jan 14;70(4):1255–1265. doi: 10.1093/jxb/erz005

Fig. 5.

Fig. 5.

Influence of carbon source and NPA treatment on the accumulation of starch in etiolated hypocotyls of exo70A1 and sec15b. (A) Hypocotyls of representative 5-day-old etiolated exo70A1 and sec15b seedlings grown on media with sucrose or mannitol and with or without 5 µM NPA addition, stained for starch. No starch accumulation was observed in WT hypocotyls under these conditions. Black arrows, collet hairs; red arrows, ectopic collet hair-like structures. (B) Quantitative comparison of starch signal intensity in etiolated hypocotyls of exo70A1 and sec15b seedlings grown on sucrose-containing media with and without NPA. Starch content was below detection limit in mannitol-grown plants. The effect of NPA in sucrose-grown plants is significant in both mutants (sec15b: Wilcoxon test P<0.003, n=10; exo70A1: Wilcoxon test P<0.03, n=8). (C) Effect of NPA treatment on the distance of ectopic collet-like structure from the true collet in the hypocotyl of exo70A1. The difference is statistically highly significant (Wilcoxon test P<0.0002, n=8 for each treatment).