Fig. 7.

Effect of IAA and 4-Cl-IAA on DR5::GUS activity in pea fruits. (A–I) Representative micrographs of GUS-stained fruit cross-sections and (J) GUS enzyme activity as detected by MUG assays. Fruits at 2 d after anthesis from plants expressing DR5::GUS were split and deseeded (split pericarp no seeds, SPNS). Twelve h after this treatment, pericarps were treated with (A–C) aqueous 0.1% Tween 80 (SPNS, controls), (D–F) IAA, or (G–I) 4-Cl-IAA. Samples were harvested at 2 h, 8 h, or 12 h after hormone application. Scale bars are 500 µm. GUS enzyme activities at the 2-h time point were as follows: intact, 26.6±3.4; SPNS, 40.1±6.4; IAA, 153.0±21.8; 4-Cl-IAA, 267.2±89.6. Data are means (±SD), n=3.