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. 2019 Feb 11;8:e43938. doi: 10.7554/eLife.43938

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© 2019, Jagannathan et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 3. — (A) Deconvolution microscopy of Drosophila larval neuroblasts in early prophase expressing GFP-Prod (green) under the control of wor-gal4 and stained with pH3 (Ser10) (red). Arrowheads in the inset indicate two of the four Prod loci while the arrow indicates Prod threads connecting the two loci. (B) Schematic depicting how homologous chromosomes separate into distinct ‘territories’ in spermatocytes in preparation of meiotic reductional division. One territory contains the X, Y, and 4^th chromosomes, a second territory contains the 2^nd chromosomes, and a third territory contains the 3^rd chromosomes. (C) FISH against the {AATAACATAG}_n satellite (red) in spermatocytes expressing GFP-Prod (green) under the control of bam-gal4 and stained with DAPI (blue). Yellow line demarcates chromosome 2 and chromosome 3 territories. Arrow indicates chromatin thread linking the two territories that is positive for DAPI, Prod, and the {AATAACATAG}_n satellite DNA. (D) FISH against the dodeca satellite (red) and the {AACAC}_n satellite (blue) in spermatocytes GFP-Prod (green) under the control of bam-gal4 and stained with DAPI (white). Yellow line demarcates chromosome 2 and chromosome 3 territories. Arrowhead indicates chromatin thread linking the two territories that is positive for DAPI and Prod. Arrows indicate location of {AACAC}_n satellite DNA that is specific to chromosome 2 and dodeca satellite DNA that is specific to chromosome 3. All scale bars are 5 μm.

Figure 3—figure supplement 1. — (A) Deconvolution microscopy of Drosophila larval neuroblasts in early prophase expressing GFP-Prod (green) under the control of wor-gal4 and stained with pH3 (Ser10) (red). Arrowheads in the inset indicate two of the four Prod loci while the arrow indicates Prod threads connecting the two loci. (B) Schematic depicting how homologous chromosomes separate into distinct ‘territories’ in spermatocytes in preparation of meiotic reductional division. One territory contains the X, Y, and 4^th chromosomes, a second territory contains the 2^nd chromosomes, and a third territory contains the 3^rd chromosomes. (C) FISH against the {AATAACATAG}_n satellite (red) in spermatocytes expressing GFP-Prod (green) under the control of bam-gal4 and stained with DAPI (blue). Yellow line demarcates chromosome 2 and chromosome 3 territories. Arrow indicates chromatin thread linking the two territories that is positive for DAPI, Prod, and the {AATAACATAG}_n satellite DNA. (D) FISH against the dodeca satellite (red) and the {AACAC}_n satellite (blue) in spermatocytes GFP-Prod (green) under the control of bam-gal4 and stained with DAPI (white). Yellow line demarcates chromosome 2 and chromosome 3 territories. Arrowhead indicates chromatin thread linking the two territories that is positive for DAPI and Prod. Arrows indicate location of {AACAC}_n satellite DNA that is specific to chromosome 2 and dodeca satellite DNA that is specific to chromosome 3. All scale bars are 5 μm.