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. 2019 Feb 20;5(2):eaat3210. doi: 10.1126/sciadv.aat3210

Fig. 3. Effect of conditioned context on the expression of Arc in BLA projection neurons to the PrL.

Fig. 3

(A) Top: Arc-positive neurons in the BLA in saline + saline, saline + naloxone, morphine + saline, and morphine + naloxone groups (red-colored). Middle: FG-labeling neurons in the BLA in saline + saline, saline + naloxone, morphine + saline, and morphine + naloxone groups (blue-colored). Bottom: Colabeling neurons of Arc and FG in the BLA in saline + saline, saline + naloxone, morphine + saline, and morphine + naloxone groups (purple-colored). Scale bars, 100 μm. BLA regions enclosed by white boxes were shown in a higher magnification in upper right square images. Scale bars, 20 μm. (B) Left: Average percentage of the coexpression of Arc and FG relative to FG in the BLA in saline + saline, saline + naloxone, morphine + saline, and morphine + naloxone groups (n = 6 mice in each group; one-way ANOVA, P > 0.05). Right: Arc-positive neurons/mm2 in the BLA in saline + saline, saline + naloxone, morphine + saline, and morphine + naloxone groups (n = 6 mice in each group; one-way ANOVA, *P < 0.05). Data are means ± SEM.