Figure 5.

Normal Thymocyte Development, but Defective Peripheral Homeostasis in the Absence of RIPK1 Expression

Ripk1^fx/fxCd4^cre and cre⁻ littermates were analyzed at 12 and 32 weeks of age.

(A) Phenotype of the indicated thymocyte populations (columns) from cre⁺ and cre^–Ripk1^fx/fx mice at 12 weeks of age, displayed as 2D plots of relative fluorescence of the indicated marker.

(B) Total cell numbers of thymocytes and the indicated thymic subsets recovered from cre⁺ (n = 5) and cre^– (n = 5) Ripk1^fx/fx mice.

(C) Total numbers of CD4⁺ and CD8⁺ naive T cells recovered from spleens of cre⁺ (n = 3–5) and cre^– (n = 3–5) Ripk1^fx/fx mice. ^∗∗∗p < 0.001 by two-way ANOVA.

(D) Histograms of relative fluorescence of IL-7R by the indicated lymph node T cell subsets from representative cre⁺ and cre^–Ripk1^fx/fx mice. Average IL-7R expression by the indicated thymic DP and SP (HSA^hi and HSA^lo) subsets and peripheral naive populations of CD4⁺ and CD8⁺ lineage cells.

Data are the pool of two independent experiments. Error bars indicate SD.